Molecular diversity of Archaea in Amazonian River sediments and characterization of cultured methanogenic species. / Diversidade molecular de arqueias em sedimentos de rios da Amazônia e caracterização de espécies metanogênicas cultivadas.
AUTOR(ES)
Ana Carolina Vieira Araujo
DATA DE PUBLICAÇÃO
2010
RESUMO
High positive fluxes of methane to the atmosphere have been detected in the Amazonian region. Methane is the second most important greenhouse gas and microorganisms belonging to the Archaea domain are responsible for approximately 70% of the total methane emitted to the atmosphere annually. The objective of this work was to characterize the Archaea diversity in two sites at Madeira and Floresta rivers sediments using molecular techniques and the culturing of methanogenic archaea. Most sequences obtained in the libraries from both rivers belonged to the Crenarchaeota domain, and around half of them presented less than 97% of similarity to sequences available in databases, revealing the existence of new archaea groups yet to be described in the literature. Cells belonging to the Methanosarcinaceae and Methanobacteriaceae families were detected in the enrichment cultures from Madeira River through the use of RNA fluorescent probes. Strains of Methanosarcina sp. and Methanobacterium sp. are being maintained under laboratory conditions. The great diversity of uncultured Archaea found emphasizes the need to study this group, mainly its physiology and, consequently, its role in the diverse environments they occupy.
ASSUNTO(S)
arqueias metanogênicas diversidade microbiana fluvial sedimentology amazônia archaea domain microbiology water sedimentologia fluvial células eucarióticas microbiologia da água biodiversity biodiversidade eukaryotic cells rio madeira domínio arqueia madeira river methanogenic archaea microbiology environmental genética molecular microbiologia ambiental amazon microbial diversity molecular genetics
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