Molecular cloning of the a1 locus of Zea mays using the transposable elements En and Mu1
AUTOR(ES)
O'Reilly, Catherine
RESUMO
The a1 locus of Zea mays has been cloned using transposable elements as gene tags. The strategy was to make genomic libraries from maize stocks with a1 mutations induced either by En(Spm) or by Robertson's Mutator-system. These libraries were then screened with either Spm-I8 and En1, for the En-containing mutant, or with Mu1 for the Mu-induced mutation. There are many En and Mu1 hybridizing sequences present in the maize genome, however, by a process of cross-screening of the positives from the two libraries and by molecular analysis of the En-positive clones it was possible to identify clones in both libraries carrying all or part of the a1 gene.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=554274Documentos Relacionados
- Influence of transposable elements on the structure and function of the A1 gene of Zea mays
- Molecular and Genetic Characterization of Mu Transposable Elements in Zea Mays: Behavior in Callus Culture and Regenerated Plants
- Nucleotide sequence of the maize transposable element Mu1
- Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays
- Somatic excision of the Mu1 transposable element of maize.