Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays

AUTOR(ES)

Pereira, Andy

RESUMO

A newly isolated, unstable mutation wx-844::En-1 of Zea mays was proven to be caused by the insertion of the autonomous transposable element En into the Waxy (Wx) gene. Molecular analysis revealed that En-1 is 8.4 kb long, has a 13-bp long perfect inverted repeat at its termini and generates a 3-bp target site duplication. En-1 is integrated into an intron located approximately in the middle of the transcribed region of the Wx gene. Structural evidence is presented indicating that a receptor component (Inhibitor) can arise by internal deletion of an autonomous En element.

Documentos Relacionados

• Molecular interactions between the components of the En-I transposable element system of Zea mays
• The Spm (En) transposable element controls the excision of a 2-kb DNA insert at the wxm-8 allele of Zea mays
• The defective En-I102 element encodes a product reducing the mutability of the En/Spm transposable element system of Zea mays
• Genetic Analysis of the Functions of the Transposable Element En in Zea Mays: Limited Transposase Elicits a Differential Response on Reporter Alleles
• Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins.