Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes
AUTOR(ES)
Marinho, Fernanda de Aquino, Gonçalves, Keyla Cristiny da Silva, Oliveira, Selma Soares de, Oliveira, Ana-Carolina de Siqueira Couto de, Bellio, Maria, d'Avila-Levy, Claudia Masini, Santos, André Luis Souza dos, Branquinha, Marta Helena
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2011-06
RESUMO
In the current study, we evaluated the mechanism of action of miltefosine, which is the first effective and safe oral treatment for visceral leishmaniasis, in Leishmania amazonensis promastigotes. Miltefosine induced a process of programmed cell death, which was determined by the externalization of phosphatidylserine, the incorporation of propidium iodide, cell-cycle arrest at the sub-G0/G1 phase and DNA fragmentation into oligonucleosome-sized fragments. Despite the intrinsic variation that is detected in Leishmania spp, our results indicate that miltefosine causes apoptosis-like death in L. amazonensis promastigote cells using a similar process that is observed in Leishmania donovani.
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