Microarray analysis of gene expression in the inflammatory T cell response and apoptosis pathways of periodontitis and non-periodontitis sites / Análise por Microarray da expressão genética de mecanismos relacionados à apoptose, resposta de células T e inflamação em sítios com e sem periodontite.

AUTOR(ES)

Marinele Ribeiro de Campos

DATA DE PUBLICAÇÃO

2007

RESUMO

It is widely believed that pathogenic bacteria provide the initial trigger in periodontal disease. However, the exact pathogenic mechanisms responsible for the progression of periodontal disease remain unclear. Moreover, the biologic basis dictating the susceptibility to disease has not been elucidated. Therefore, the present study sought to investigate the expression of genes altered in periodontal disease sites. To that aim, a focused microarray system was utilized to examine the expression of 288 genes related to apoptosis, T cell response and inflammation. Total RNA was extracted from gingival tissue and peripheral blood of periodontitis (N=10) and nonperiodontitis (N=4) subjects. The RNA was converted to cRNA, labeled and hybridized to oligonucleotide microarray plates. Following exposure, the positively labeled genes were quantified and the level of expression within periodontitis gingival was compared to the PB of the same subjects, as well as with GT of NP subjects. To validate the results the selected genes (p

ASSUNTO(S)

periodontal disease t cell apoptosis células t inflamação microarray doença periodontal inflammation apoptose microarray

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