Membrane filter method for enumerating Escherichia coli.
AUTOR(ES)
Dufour, A P
RESUMO
A membrane filter procedure for enumerating Escherichia coli was developed and evaluated. The method quantifies E. coli within 24 h without requiring subculture and identification of isolates. It incorporates a primary selective-differential medium for gram-negative, lactose-fermenting bacteria; resuscitation of weakened organisms by incubation for 2 h at 35 degrees C before incubation at 44.5 degrees C for 18 to 22 h; and an in situ urease test to differentiate E. coli from other thermotolerant, lactose-positive organisms. The recovery of E. coli from marine, estuarine, and freshwater samples exceeded 90%. Of the presumptively positive colonies, 91% were verified as E. coli. Less than 1% of all of the verified E. coli colonies failed to react typically.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=243882Documentos Relacionados
- Two-temperature membrane filter method for enumerating fecal coliform bacteria from chlorinated effluents.
- Rapid screening method for enterotoxigenic Escherichia coli.
- Improved detection of coliforms and Escherichia coli in foods by a membrane filter method.
- Application of a DNA Hybridization–Hydrophobic-Grid Membrane Filter Method for Detection and Isolation of Verotoxigenic Escherichia coli
- Quantitative method for enumeration of enterotoxigenic Escherichia coli.
