Quantitative method for enumeration of enterotoxigenic Escherichia coli.
AUTOR(ES)
Calderon, R L
RESUMO
A rapid method was developed to quantify toxigenic Escherichia coli, using a membrane filter procedure. After filtration of samples, the membrane filter was first incubated on a medium selective for E. coli (24 h, 44 degrees C) and then transferred to tryptic soy agar (3%; 6 h, 37 degrees C). To assay for labile toxin-producing colonies, the filter was then transferred to a monolayer of Y-1 cells, the E. coli colonies were marked on the bottom of the petri dish, and the filter was removed after 15 min. The monolayer was observed for a positive rounding effect after a 15- to 24-h incubation. The method has an upper limit of detecting 30 toxigenic colonies per plate and can detect as few as one toxigenic colony per plate. A preliminary screening for these enterotoxigenic strains in polluted waters and known positive fecal samples was performed, and positive results were obtained with fecal samples only.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=273736Documentos Relacionados
- Rapid screening method for enterotoxigenic Escherichia coli.
- Lactobacillus prophylaxis for diarrhea due to enterotoxigenic Escherichia coli.
- Antibiotic resistance in Enterotoxigenic and non-enterotoxigenic Escherichia coli.
- Hemolytic activity in enterotoxigenic and non-enterotoxigenic strains of Escherichia coli.
- Clinical cholera caused by enterotoxigenic Escherichia coli.