MECHANISM OF DNA CHAIN GROWTH, IV. DIRECTION OF SYNTHESIS OF T4 SHORT DNA CHAINS AS REVEALED BY EXONUCLEOLYTIC DEGRADATION*
AUTOR(ES)
Okazaki, Tuneko
RESUMO
T4 nascent short chains labeled at their growing ends with H3-thymidine and uniformly with C14-thymidine were prepared, separated into complementary strands, and degraded by E. coli exonuclease I in the 3′ to 5′ direction or by B. subtilis nuclease in the 5′ to 3′ direction. The kinetics of release of H3 and C14 labels by both enzymes was consistent with the conclusion that the H3 label is at the 3′ end of the nascent short chains of both strands and that the short chains are products of discontinuous synthesis in the 5′ to 3′ direction along the two template strands.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=223275Documentos Relacionados
- MECHANISM OF DNA CHAIN GROWTH, III. EQUAL ANNEALING OF T4 NASCENT SHORT DNA CHAINS WITH THE SEPARATED COMPLEMENTARY STRANDS OF THE PHAGE DNA*
- Mechanism of DNA chain growth, II. Accumulation of newly synthesized short chains in E. coli infected with ligase-defective T4 phages.
- MECHANISM OF DNA CHAIN GROWTH, II. ACCUMULATION OF NEWLY SYNTHESIZED SHORT CHAINS IN Escherichia coli INFECTED WITH LIGASE-DEFECTIVE T4 PHAGES
- Biochemistry of DNA-Defective Amber Mutants of Bacteriophage T4 IV. DNA Synthesis in Plasmolyzed Cells
- Marker-Dependent Recombination in T4 Bacteriophage. IV. Recombinational Effects of Antimutator T4 DNA Polymerase