MECHANISM OF DNA CHAIN GROWTH, III. EQUAL ANNEALING OF T4 NASCENT SHORT DNA CHAINS WITH THE SEPARATED COMPLEMENTARY STRANDS OF THE PHAGE DNA*

AUTOR(ES)

Sugimoto, Kazunori

RESUMO

Nascent short DNA chains isolated from T4-infected E. coli under a variety of conditions anneal equally to the separated complementary phage DNA strands. The samples examined include: pulse-labeled short chains isolated by alkaline sucrose gradient sedimentation from the T4D (wild type)-infected cells in both the early and late stages of phage DNA synthesis; nascent chains accumulated during ligase inhibition of T4 ts B20-infected cells; and the single-stranded nascent short chains isolated from T4D-infected cells by mild procedures involving no denaturation treatment. The results are consistent with the hypothesis that both strands of DNA are synthesized discontinuously.

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