Mapeamento fÃsico e anÃlise evolutiva em Phaseolus vulgaris L. e P. lunatus L., utilizando hibridaÃÃo in situ fluorescente(FISH)

AUTOR(ES)

CÃcero Carlos de Souza Almeida

DATA DE PUBLICAÇÃO

2006

RESUMO

This work presents the analyses of the heterochromatin distribution and number of 5S and 45S rDNA sites in the species P. lunatus and P. vulgaris and the development of a cytogenetic map of P. lunatus based on the localization of two bacteriophages and 30 BAC (Bacterial Artificial Chromosome) of chromosomes 3, a and 7 from P. vulgaris. In both species, the constitutive heterochromatin was preferentially located in the pericentromeric regions of most chromosomes. A high variation in the number of 45S rDNA sites was observed in P. vulgaris. In this species three to four sites were observed in Mesoamerican accessions and from six to nine sites in the Andean ones. The number of 5S rDNA sites was stable with two sites bring observed in all accession. In contrast, the number of 45S rDNA sites in P. lunatus remained constant, with a single locus bring observed, while the 5S rDNA was observed forming a single locus in many accessions, but has a duplicated in others. In situ hybridization of 30 BACs and two bacteriophages from P. vulgaris in P. lunatus revealed the presence of repetitive DNA in 7 BACs, while could therefore not be mapped. The remaining BACs and phages were shown to be conserved among the species, because they were mapped in similar positions in P. vulgaris genetic map and P. lunatus cytogenetic map. These results confirm the similarity between the karyotypes of both species and suggest that no make chromosomal rearrangement occurred since the time of divergence. The differencial amplification of 45S rDNA and other repetitive sequences are probably the main karyotypical differences among the species, because some sequences have been detected in P. vulgaris but not in P. lunatus and other seem to be present in P. lunatus but are apparently absent from P. vulgaris

ASSUNTO(S)

ciencias biologicas phaseolus evolution mapeamento fÃsico physic mapping evoluÃÃo phaseolus

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