Limonóide de guarea kunthiana com potencial leishmanicida

Raquel Cosmo de Lima

Leishmaniasis is a neglected disease that affects many countries and is a severe public health problem. The number of cases has been increasing mainly due to Leishmania-HIV co-infection. The clinic forms include tegumentar (cutaneous, diffuse cutaneous and muco-cutaneous leishmaniasis), which affects skin and mucous membrane, and visceral (kala-azar), which affects internal organs. The available therapies include antimony, pentamidine and anphoterin B, which cause serious side effects, as well as resistance cases. Interest in investigating new active molecules with lower toxicity include compounds extracted from vegetal species. Towards this end, we performed a Bank Extract screening against Leishmania (Leishmania) amazonensis. The hexanic extract of Meliaceae family Guarea kunthiana root wood showed IC100 of 125 g/mL. The active extract bioguided fractionating in the open silica chromatographic column produced 19 groups (G). The most active were G1 (IC100 = 75 μg/mL), G5 (IC100 = 18,75 μg/mL), G8 (IC100 = 75 μg/mL) and G17 (IC100 = 37.5 μg/mL). Until now, we have promising results for G17. G17 fractionating allowed 22,24diepoxitirucall-7-en-3-one limoid triterpene isolation, with leishmanicidal potential. This compound was elucidated by spectrometric methods of NMR (1H NMR, 13C NMR, COSY, HSQC, HMBC, NOESY), including Tandem mass spectrometry (EM-EM). To the best of our knowledge, 22,24diepoxitirucall-7-en-3-one limonoid is unpublished in Guarea spp. and Meliaceae family, nor is there information regarding limonoids action against Leishmania. Thus, 22,24diepoxitirucall-7-en-3-one limonoid is promising for new studies, towards the identification alternative options for leishmaniasis control.

Limonóide de guarea kunthiana com potencial leishmanicida

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