Isolation and molecular size of Clostridium botulinum type C toxin.

AUTOR(ES)

Syuto, B

RESUMO

A procedure is described for the purification of hemagglutinin-free Clostridium botulinum type C toxin. The toxin was purified approximately 1,000-fold from the original culture supernatant in an overall yield of 60% to a final specific toxicity of 4.4 x 10(7) minimal lethal doses/mg of protein. The toxin had a molecular weight of 141,000 and consisted of a heavy and a light chain. The molecular weights of the subunits were approximately 98,000 and 53,000. When comparing the molecular size and composition of type C toxin to that of botulinum toxins of different types, some common features may be suggested; i.e., the toxin has a molecular weight between 141,000 to 160,000 and is comprised of a heavy and a light chain linked by disulfide bonds (or bond).

Documentos Relacionados

• Monoclonal antibody to type F Clostridium botulinum toxin.
• Amino acid composition of Clostridium botulinum type A toxin.
• Antigenicites of fragments of Clostridium botulinum type B derivative toxin.
• Simplified purification method for Clostridium botulinum type E toxin.
• Immunological response to Clostridium botulinum toxin.