Monoclonal antibody to type F Clostridium botulinum toxin.

AUTOR(ES)

Ferreira, J L

RESUMO

Hybridomas synthesizing monoclonal antibodies (MAbs) against type F Clostridium botulinum toxin were developed. MAb from one stable hybridoma, hybridoma 223, consisted of kappa light chains and an immunoglobulin G subclass 2a heavy chain. This MAb was used in a double-sandwich enzyme-linked immunosorbent assay to detect type F toxin in foods, culture fluids, and purified toxin preparations. The sensitivity of the double-sandwich enzyme-linked immunosorbent assay was approximately 10 mouse lethal doses of toxin per ml of toxic fluid.

Documentos Relacionados

• Immunological response to Clostridium botulinum toxin.
• The use of monoclonal antibodies to analyze the structure of Clostridium botulinum type E derivative toxin.
• Simplified purification method for Clostridium botulinum type E toxin.
• Amino acid composition of Clostridium botulinum type A toxin.
• Establishment of a monoclonal antibody recognizing an antigenic site common to Clostridium botulinum type B, C1, D, and E toxins and tetanus toxin.