Isolation and Mapping of Phosphotransferase Mutants in Escherichia coli
AUTOR(ES)
Epstein, Wolfgang
RESUMO
Mutants of Escherichia coli K-12 defective in enzyme I or Hpr, the two common components of the phosphoenolpyruvate-dependent phosphotransferase system, were isolated by a simple, direct method. The ptsI locus, the structural gene for enzyme I, and the ptsH locus, the site of mutations leading to loss of Hpr activity, are adjacent genes and could be part of a single operon. These two genes lie between the purC and supN markers in the order: strA... guaB-purC-ptsI-ptsH-supN-dsdA... his.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=285060Documentos Relacionados
- Isolation and Mapping of Polynucleotide Phosphorylase Mutants of Escherichia coli
- Isolation, characterization, and mapping of Escherichia coli mutants blocked in the synthesis of ornithine decarboxylase.
- Isolation, genetic mapping, and characterization of Escherichia coli K-12 mutants lacking gamma-glutamyltranspeptidase.
- Lack of glucose phosphotransferase function in phosphofructokinase mutants of Escherichia coli.
- Isolation and characterization of isoprene mutants of Escherichia coli.
