Isolation, genetic mapping, and characterization of Escherichia coli K-12 mutants lacking gamma-glutamyltranspeptidase.
AUTOR(ES)
Suzuki, H
RESUMO
Escherichia coli K-12 mutants lacking gamma-glutamyltranspeptidase (EC 2.3.2.2) were isolated after mutagenesis of cells with ethyl methanesulfonate. They lost the enzyme activity to different extents. The mutations of two mutants that had lost the enzyme activity completely were mapped at 76 min of the E. coli K-12 linkage map. These mutations made the cells neither nutrient requiring nor cold sensitive. The mutants leaked much more glutathione into the medium than the wild type. We propose the symbol ggt for these mutations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=213689Documentos Relacionados
- DNA sequence of the Escherichia coli K-12 gamma-glutamyltranspeptidase gene, ggt.
- gamma-Glutamyltranspeptidase from Escherichia coli K-12: purification and properties.
- gamma-Glutamyltranspeptidase from Escherichia coli K-12: formation and localization.
- Characterization and sequence of a cDNA clone of gamma-glutamyltranspeptidase.
- A new pleiotropic mutation causing defective carbohydrate uptake in Escherichia coli K-12: isolation, mapping, and preliminary characterization.