Isolated horizontal cells from carp retina demonstrate dopamine-dependent accumulation of cyclic AMP.
AUTOR(ES)
Van Buskirk, R
RESUMO
Horizontal cells of the carp retina were separated from other retinal cell types by using enzymatic dissociation and velocity sedimentation at unit gravity. Fractions containing horizontal cells were tested for their ability to accumulate cyclic AMP in the presence of various putative neurotransmitters. Micromolar concentrations of dopamine, when added in the presence of 3-isobutyl-1-methylxanthine, stimulated cyclic AMP accumulation in these isolated cells. The dopamine-dependent accumulation of cyclic AMP in intact isolated horizontal cells was blocked by nanomolar concentrations of dopamine antagonists such as haloperidol, (+)-butaclamol, and fluphenazine. The results indicate that there is a postsynaptic dopamine receptor on carp horizontal cells that is associated with adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1].
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=349364Documentos Relacionados
- Age-Related Dopamine-Dependent Disorders
- Decoupling of horizontal cells in carp and turtle retinae by intracellular injection of cyclic AMP.
- Dopamine-dependent responses to morphine depend on glucocorticoid receptors
- Dopamine-dependent synaptic plasticity in striatum during in vivo development
- Quantitative correlation of dopamine-dependent adenylate cyclase with responses to levodopa in various mice.
