Influencia dos polimeros poli caprolactona (PCL) e poli L-acido latico (PLLA), sobre a expressão de componentes da membarna de celulas de Schwann in vitro e in vivo / Influence of poly caprolactone (PCL) and poly L-lactic acid (PLLA) polymers on Schwann cell basal lamina components expression in vitro and in vivo

AUTOR(ES)

Amauri Pierucci

DATA DE PUBLICAÇÃO

2007

RESUMO

The present study proposed a new approach to produce tubular conduits designed for peripheral nerve repair. In this sense, membranes of PLLA and PCL were obtained after solvent evaporation and wrapped around a mandrel. The effectiveness of the nerve regeneration was compared with polyethylene and PCL extruded prosthesis 30 and 60 days after surgery. The comparison between extrusion and solvent tubes cleared shown structural differences which were directly proportional to the hardness and transparency. An important factor to be considered is that the fiber counting indicated that solvent PCL tubes provided a significantly greater number of axons 30 days after repair. Sixty days after operation, the greatest regenerative performance was obtained with PCL, regardless the method of construction of the tube. An intense labeling against S-100, type IV collagen and laminin could be observed in the tissue obtained from solvent PCL and PLLA groups, indicating that such constructions are able to positively stimulate Schwann cell responses. Overall, the present results provide evidence that solvent conduits may be regarded as an alternative preparation method for tubular prosthesis aiming peripheral nerve regeneration. In the in vitro study, PCL and PLLA solvent polymers were used for culturing purified of Schwann cells. The imunolabeling revealed an up-regularion of the expression of collagen IV, laminin I, laminin II and S-100 by the Schwann cells, showing that biodegradable polymers enhance the activity of such cells, positively influencing the peripheral nerve regeneration process.

ASSUNTO(S)

schwann cells schwann extracellular matrix celulas de regeneração perifericas matriz extracelular biomaterials biomateriais peripheral regeneration

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