Influencia da ciclosporina A na produção e atividade enzimatica de metaloproteinases de matriz no processo de reparação alveolar de molares de ratos Wistar / Action of cyclosporin A on the production and activity of matrix metalloproteinases during the alveolar wound healing after rats Wistar s molar extraction

AUTOR(ES)
DATA DE PUBLICAÇÃO

2000

RESUMO

Cyclosporin A (CSA) is a potent immunosuppressive agent which acts in a specific and reversible way, mainly in T CD4 Iymphocytes. The aim of this study was to investigate the production and activity of matrix metalloproteinases (MMPs) through zymography during alveolar wound healing of inferior molars of Wistar s rats treated with CSA. Thirty Wistar rats where daily injected subcutaneously with 10 mg/kg/body weight of CSA starting seven days before the extraction and continuing from 1 to 10 days after surgery. The same numbers of rats were injected with the same volume of saline solution as a control group. Specific inhibitors were used to identify MMPs enzymes group. The data obtained from different healing periods, showed secretion and gelatinolytic activity, mainly by MMP-9 and MMP-2. The total gelatinolytic activity of MMP-9 and MMP-2 of the cell culture medium incubated with granulation tissue from the healing area, were lower in the CSA group than in the control group. The results showed that CSA use reduced the MMPs enzyme activity, which may have an important role in the alveolar wound healing process

ASSUNTO(S)

rats metaloproteinas ciclosporina metalloproteinases rato cyclosporin alveolar wound healing

ACESSO AO ARTIGO

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