Influence of indole-3-acetic acid supplementation on the bacterial killing and cellular integrity on rat’s neutrophils / Influência do ácido indol-3-acético na capacidade fagocitária e na integridade celular de neutrófilos de ratos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Indole acetic acid is (IAA) a hormone termed auxins and there is a direct correlation between the cytotoxic effect of IAA and the peroxidase activity of the animal cells. Neutrophils present a higher peroxidase activity and the IAA leads to marked ultra structural changes and death on these cells in culture. However studies in vivo show that IAA administration at low doses does not promoting a prooxidant effect on neutrophil and this supplementation to increase of the engulfment of Zymosan particles by these cells. In present study were evaluated the effect of IAA administration in the phagocytic capacity and cellular integrity on rat’s neutrophils from the following parameters: i) phagocytic capacity – engulfment and killing of the Staphylococcus aureus; ii) cellular integrity – plasma membrane integrity, DNA fragmentation and mitochondrial transmembrane potential and iii) myeloperoxidase activity. The IAA treatment imposed did not show another significant alteration in the S. aureus engulfment and killing capacity by neutrophils to compare with controls. The cellular integrity and myeloperoxidase activity in the neutrophils did not have alteration by IAA supplementation to compare with the controls. In conclusion the IAA administration at low doses did not show effective action in S. aureus killing by neutrophils and the same time as did not alter the cellular integrity and myeloperoxidase activity by this cell.

ASSUNTO(S)

fagocitose staphylococcus aureus myeloperoxidase fragmentação de dna auxina dna fragmentation mieloperoxidase staphylococcus aureus phagocytosis auxin

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