InfluÃncia da infecÃÃo pelo lentivÃrus Caprine arthritis â encephalitis vÃrus nos lipÃdeos plasmÃticos de caprinos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Caprine arthritis-encephalitis virus (CAEV) infection is a progressive chronic disease characterized by arthritis, lesions in lymphoid tissue, nervous system and in other organs, weight loss, weakness and death of the animals. The present study aimed to evaluate the cholesterol, phospholipids and triglycerides levels in caprine flock from Pernambuco/Brazil. Plasma of animals was analyzed by immunological method (ELISA) for the presence of lentivirus, and it was selected animals infected by CAEV without clinical signals. The control group was selected from health flocks from the same region and similar management condition. Plasma low density lipoprotein (LDL) and very low density lipoprotein (VLDL) were precipitated from high density lipoprotein (HDL) by the addition of 1.4 mM sodium phosphotungstate with 53.0 mM magnesium chloride. Neutral lipids and phospholipid subclasses were separated by thin layer chromatography. Plasma cholesterol, HDL cholesterol (HDL-C) and triglycerides were analyzed by enzymatic assays, whilst VLDL cholesterol (VLDL-C) and LDL cholesterol (LDL-C) was measured by Friedwald Equation. The total phospholipids and its subclasses were measured by chemical method. Animals infected by CAEV showed significant increase in total cholesterol, cholesteryl ester, total phospholipids and LDL-C. However, the concentration of triglycerides, HDL-C and VLDL-C were similar to those found for the control group. The phospholipid subclasses, lysophosphatidylcholine, phosphatidylcholine and phosphatidyletanolamine, were significantly higher in plasma from infected animals than in plasma of the control group. However, sphingomyeline level in plasma of infected animals appears similar to those from control group. The results show that the increase in plasma total cholesterol and in the main phospholipid subclasses concentrations in CAEV. The increase in plasma phospholipids found in the CAEV infected goats may lead to an abnormal composition of cell membrane, and consequently may alter the cell function. Furthermore, the increase in low density lipoprotein-cholesterol in animals infected by CAEV could predispose this group to hypercholesterolemia and to coronary heart disease, and this may have a clinical relevance, particularly, to those goats post-parturition and/or to those involved in the milk production

ASSUNTO(S)

lentivÃrus caprine arthritis bioquimica caprinos infecÃÃo

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