InfluÃncia da fonte de carbono na produÃÃo de biosurfactante por espÃcies de Pseudomonas isoladas de efluente agroindustrial

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

The biosurfactantes possess many advantages in relation to the synthetic, surfactants, such as: low toxicity, biodegradability, great chemical and structural diversity. The objective of were: 1) To isolate bacteria of Pseudomonas genus in agri-industrial effluent; 2) To detect the production in vitro of the rhamnolipidic biosurfactant; 3) To verify the use of maize oil in the production of biosurfactant in liquid culture and 4) To characterize partially the biosurfactant produced on maize oil for thin layer chromatography (TLC). Three bacteria of the Pseudomonas genus were isolated effluent hamming from the laudering of sugar cane of the SA Coruripe Sugar and Alcohol (Alagoas/Brazil) and identified in species level, using kit API20E (BioMÃrieux, France), as being P. aeruginosa, P. putida and P. fluorescens. The Siegmund &Wagner medium was used to characterize the producing strains of rhamnolipid being the production of this biosurfactante in vitro evidenced only for the P. aeruginosa species. The production of rhamnolipid in liquid medium for the isolated P. aeruginosa using maize oil revealed the highest concentration (200.58 mg/L) with 120 h incubation, when the stationary phase revealed as carbon source clear, even so no alteration accented of pH has been verified. The biosurfactante also was characterized thin layer chromatography that was possible to be detected and the Rf values of been similar to those described in literature for the different structural forms of rhamnolipid

ASSUNTO(S)

biosurfactante bioquimica pseudomonas biosurfactante pseudomonas

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