Imunogenicidade da vacina de DNA quimérica LAMP-1/p55Gag do HIV-1 no período neonatal e efeito da imunização materna na resposta vacinal da prole de camundongos. / Immunogenicity of LAMP-1/p55Gag of HIV-1 DNA chimeric vaccine on neonatal period and effect of maternal immunization immune response of vaccinated offspring.
AUTOR(ES)
Paula Ordonhez Rigato
DATA DE PUBLICAÇÃO
2009
RESUMO
The immunogenicity of LAMP/gag DNA vaccine of HIV-1, which target the antigen to the class II MHC compartment, was evaluated on BALB/c neonate mice and also the effect of maternal immunization on off spring vaccine response was analyzed. The LAMP/gag neonatal vaccination elicited IgG antibody anti-Gag production and augment the IFN-g producing cells to the MHC class I and II epitopes; CD8+ T cells expressing TNF-a, IFN-g, granzime and class I immunodominant epitope of Gag specific cells. Moreover of generate long lasting humoral and cellular immune response until 9 months of age. The LAMP/gag pre-conceptional immunization transferred IgG1 antibody anti-Gag to offspring (by transplacentary and breastmilk route), diminished humoral/cellular response, inducing CD4+CD25+FoxP3 cells on immunized offspring, however did not affect the memory response. Strategically, the intravenous immunization during gestation avoided the antibody transference and primed the fetal immune system.
ASSUNTO(S)
síndrome de imunodeficiência adquirida (aids) dna hiv immunology acquired immunodeficiency (aids) neonatologia hiv vaccines vacinas imunologia neonatology dna
Documentos Relacionados
- Efeito da imunização materna com Ovalbumina na ativação de células dendríticas e geração de linfócitos T reguladores na prole de camundongos.
- HIV-1 Gag Processing Intermediates Trans-dominantly Interfere with HIV-1 Infectivity*
- Análise da imunogenicidade de uma vacina de DNA codificando epitopos CD4 promíscuos e conservados do HIV-1 em camundongos BALB/c e transgênicos para moléculas de HLA classe II
- Enhanced Presentation of Major Histocompatibility Complex Class I-Restricted Human Immunodeficiency Virus Type 1 (HIV-1) Gag-Specific Epitopes after DNA Immunization with Vectors Coding for Vesicular Stomatitis Virus Glycoprotein- Pseudotyped HIV-1 Gag Particles
- A myristoyl switch regulates membrane binding of HIV-1 Gag