Immunomodulation Properties of Solid-State Fermented Laetiporussulphureus Ethanol Extracts in Chicken Peripheral Blood Monocytes In Vitro

AUTOR(ES)

Lin, WC

FONTE

Braz. J. Poult. Sci.

DATA DE PUBLICAÇÃO

20/12/2019

RESUMO

ABSTRACT Laetiporus sp. is recognized as a fungal species traditionally used for medicinal purposes. This study investigated the in-vitro effects of solid-state fermented Laetiporussulphureus ethanol extracts (LSE) for their immunomodulatory potential. Bioactive levels detected in the LSE on different days throughout the fermentation period revealed that the 12th day was the most efficient, with 7.19 ± 0.66 GAE/g DM crude phenolic content, 2.71 ± 0.03 UAE/g DM crude triterpenoid content, 12.93 ± 0.88 GCE/g DM crude polysaccharides, and 96.44 ± 0.2 mg/g DM ergosterol content. In-vitroLSE tests on chPBMC showed no cytotoxicity within a range of 0.05-1 mg/mL, but LPS-inhibited cell viability was improved, as well as LPS-induced nitric oxide (NO) production and mRNA levels of nuclear factor kappa B (NFκB), Toll-like receptor 4 (TLR4), inducible nitric oxide synthase (iNOS), and interleukin (IL)-1βwere attenuated Furthermore, the direct application of LSE on chPBMC showed a small but not significant increase in NFκB, TLR4, and iNOS mRNA expression compared with the control group. These results indicate the potential of LSE to modulate LPS-triggered inflammation processes involving TLR4 and NFκB mediation. However, further experiments are required to determine the specific pathway.

Documentos Relacionados

• In vitro free radicals scavenging activity and antioxidant capacity of solid-state fermented wheat bran and its potential modulation of antioxidative molecular targets in chicken PBMC
• High-resolution solid-state NMR of quadrupolar nuclei
• Solid-state and mechanical properties of aqueous chitosan-amylose starch films plasticized with polyols
• Lipase production by solid-state fermentation in fixed-bed bioreactors
• Sperathe effects of solid-state fermentation in the functional properties of defatted rice bran and wheat bran