Imaging of optically active biological structures by use of circularly polarized light.
AUTOR(ES)
Keller, D
RESUMO
If an optically active (chiral) sample is placed in a microscope and illuminated with circularly polarized light, an image can be formed that is related to the circular dichroism of each feature of the sample. A theoretical investigation has been done for the circular differential image obtained by subtracting the images formed under right- and left-circularly polarized light. Two types of differential images are possible: (i) dark-field images formed from light reflected or scattered by the sample and (ii) bright-field images formed from light transmitted through the sample. The sign and magnitude of each feature in a circular differential image strongly depend on the structure of the sample. The dark-field circular differential images are most sensitive to large features with dimensions similar to the wavelength of illumination whereas the bright-field images are most sensitive to the short-range molecular order. Applications of circular differential imaging may include clinical fingerprinting of normal and transformed cells and structural analysis of individual cellular components.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=397046Documentos Relacionados
- Differential Scatter of Left and Right Circularlay Polarized Light by Optically Active Particulate Systems
- Improvement of Circularly Polarized Slot-Patch Antenna Parameters by Using Electromagnetic Band Gap Structures
- Visualization of oriented hemoglobin S in individual erythrocytes by differential extinction of polarized light.
- Time-resolved circularly polarized protein phosphorescence.
- Screening for neurofibrillary tangles and argyrophilic plaques with Congo Red and polarized light.