Identificação de seqüências expressas (EST) em embriões de Gallus gallus. / Study of expressed sequence tags (est) during chicken (Gallus gallus) embryonic development.

AUTOR(ES)

Erika Cristina Jorge

DATA DE PUBLICAÇÃO

2002

RESUMO

Embryo development requires rigorous control of expression of various genes, which may become active at an adequate time and site for the correct establishment of organ and structure of the organism. Identification of Expressed Sequence Tags (EST) allows the determination of genes expressed in specific tissues at various stages of development. To identify genes expressed during embryonic development of Gallus gallus, EST were obtained from 5 end of clones from three cDNA libraries, derived from (1) whole embryos, (2) limb buds (hindlimb and forelimb), and (3) somites and neural tube. EST sequences were analyzed using the softwares Phred, Cap3 and Consed to evaluate base quality and clustering, resulting in 4998 valid EST, according to the parameters established. All consensus sequences of clusters and singletons obtained were compared with sequences available at GenBank (http://www.ncbi.nlm.nih.gov) and classified into twelve categories according to function. Categorization revealed that about 25% of the genes were not described in the database consulted (Low and No hit). About 15% of the genes had no defined function (conserved hypothetical). The remaining sixty percent permitted identification of genes involved with somitogenesis, skeletal muscle and limb bud development in vertebrates and cellular maintenance and structure. The set of EST identified in this project resulted in a database with around 5000 EST of Gallus gallus embryonic stages, which is a new source of gene identification to facilitate investigation of regulatory processes in embryo development and identification of Single Nucleotide Polymorphisms (SNPs).

ASSUNTO(S)

development biology chicken biologia do desenvolvido genes embriogênes animal galos biologia molecular animal development molecular biology

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