Hemoglobin Switching in Sheep and Goats: Change in Functional Globin Messenger RNA in Reticulocytes and Bone Marrow Cells

AUTOR(ES)

Nienhuis, Arthur W.

RESUMO

Anemia causes a change in the type of circulating hemoglobin in goats and certain sheep: HbA (α2β2A) is replaced by HbC (α2β2C). We have isolated globin mRNA from erythroid cells of anemic and nonanemic animals to investigate the mechanism whereby anemia causes this switch. To study several stages in transition from βA to βC synthesis, active globin mRNA was isolated from bone marrow cells, as well as from reticulocytes. By assaying these globin mRNAs in a rabbit reticulocyte cell-free system, we have demonstrated that the switch from βA to βC globin synthesis is mediated via a change in functional globin mRNA.

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