Globin chain synthesis in sickle beta-thalassaemic bone marrow and reticulocytes.
AUTOR(ES)
Ladas, S
RESUMO
Globin chain synthesis was studied in reticulocytes and bone marrow erythroid precursors in four sickle beta-thalassaemic Greek patients. Significant globin chain imbalance was found in reticulocytes (alpha/gamma + beta A + beta S = 2.20 +/- SD 0.16) and bone marrow (alpha/gamma + beta A + beta S = 1.58 +/- SD 0.11) after two hours' incubation. There was evidence of contamination of the gamma, beta A, and, to a lesser extent, of the beta S chain by non-haem proteins. The contamination was more obvious in chromatograms obtained from whole cell bone marrow samples and could partially explain the lower alpha/non-alpha ratio found in bone marrow.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1012673Documentos Relacionados
- Effect of isoniazid, a haem inhibitor, on globin chain synthesis in reticulocytes from non-thalassaemic and beta thalassaemic subjects.
- Bone marrow and peripheral blood globin chain synthesis in sickle cell beta zero thalassaemia.
- Imbalanced globin chain synthesis in heterozygous beta-thalassemic bone marrow.
- Heat shock-induced translational control of HSP70 and globin synthesis in chicken reticulocytes.
- Translational profiles of alpha 1-, alpha 2-, and beta-globin messenger ribonucleic acids in human reticulocytes.