Gram Type-Specific Broad-Range PCR Amplification for Rapid Detection of 62 Pathogenic Bacteria
AUTOR(ES)
Klausegger, Alfred
FONTE
American Society for Microbiology
RESUMO
Broad-range PCR has proven to be useful for the detection of bacteria. A set of broad-range PCR primers directed against conserved regions in the 16S rRNA gene was designed to specifically amplify either gram-positive or gram-negative bacteria. The gram type-specific broad-range PCR correctly classified all 62 pathogenic species tested.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=84344Documentos Relacionados
- Gram Type-Specific Broad-Range PCR Amplification for Rapid Detection of 62 Pathogenic Bacteria
- Optimal DNA Isolation Method for Detection of Bacteria in Clinical Specimens by Broad-Range PCR
- Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing.
- Contamination Management of Broad-Range or Specific PCR: Is There Any Difference?
- A broad-range PCR technique for the diagnosis of infective endocarditis
