Globlal gene expression analysis during germination of the aquatic fungus Blastocladiella emersonii / Análise da expressão gênica global durante a germinação do fungo aquático Blastocladiella emersonii

AUTOR(ES)

Silvia Maria Salem Izacc

DATA DE PUBLICAÇÃO

2006

RESUMO

We conducted large scale cDNA sequencing from libraries obtained using RNA from germinating cells of the aquatic fungus Blastocladiella emersonii. Data obtained in this work, along with cDNAs from sporulating cells, lead to the discovery of nearly 4.900 different genes from B. emersonii (Ribichich et al., 2005). The putative genes were annotated and associated with the functional categories described by the Gene Ontology Consortium and are available at the web site http://www.blasto.iq.usp.br. The expression patterns of these genes were evaluated by digital Northern analysis and we detected several transcripts that presented expression profile regulated during germination. In a second approach, nearly 3.600 putative genes from B. emersonii were spotted into glass slides and used as probes to investigate the global gene expression pattern in cells isolated at different times after induction of germination. We also analyzed the differences between the germination triggered in nutrient medium and the germination in inorganic solution, in which the effective inducers of germination were either adenine or potassium ions. More than 900 genes were differentially expressed during germination in nutrient medium in at least one of the time points analyzed, which correspond to 26 % of the total genes in the microarrays. The genes induced during this process were mainly those involved in cellular growth, including protein biosynthesis, transcription and energetic metabolism. However, these genes were not induced when germination occurred in inorganic solution. In addition, we verified that many transcripts involved in sensing the environment and also in signal transduction, encoding proteins necessary to trigger the germination program, were present in the zoospores. Another finding is that some of the transcripts found in zoospores were repressed when germination proceeded in nutrient medium, but were maintained at high levels when germination occurred in inorganic solution, suggesting that nutrients exert an important role in regulating the expression of some genes in this stage of development.

ASSUNTO(S)

fungos blastocladiella emersonii expressão gênica gene expression microarray blastocladiella emersonii microarranjos de dna germination ests ests germinação

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