Genotoxicity Evaluation of Dipotassium -Trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), in Human Lymphocyte Cultures and Mice Reticulocytes

AUTOR(ES)

Haveric, Sanin, Hadzic, Maida, Haveric, Anja, Mijanovic, Mirjana, Hadziselimovic, Rifat, Galic, Borivoj

FONTE

Braz. arch. biol. technol.

DATA DE PUBLICAÇÃO

31/10/2016

RESUMO

ABSTRACT Genotoxic effects of inorganic molecule dipotassium-trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), a promising new therapeutic for the epidermal changes treatment, have been evaluated. In vitro analysis included evaluation of genotoxic and cytotoxic potential of K2(B3O3F4OH) in concentrations of 0.01, 0.02, 0.05 and 0.06 mg/mL applying cytokinesis-block micronucleus cytome assay in human lymphocyte culture. With the increase of concentration the frequency of micronuclei elevated but the differences were not significant. Also, there were no significant differences among the frequencies of nuclear buds and nucleoplasmic bridges between controls and treated cultures. Nuclear division index and nuclear division cytotoxycity index values did not reveal significant cytotoxic effect of K2(B3O3F4OH). In vivo genotoxic effects were analyzed on BALB/c mice applying reticulocytes micronucleus assay. K2(B3O3F4OH) was administrated intraperitoneally in final concentrations of 10, 20, 50 and 55 mg/kg. Significant decrease of reticulocytes ratio and increase of micronuclei frequencies against pre-treatments were found for both sampling periods of 48 and 72 hours of the highest applied concentration. This study confirmed that K2(B3O3F4OH) is not genotoxic in tested concentrations in vitro as well as in concentrations lower than 55 mg/kg in vivo. This study presents a reliable basis for further pre-clinical and potential clinical investigations.

Documentos Relacionados

• Comparison of replication of adenovirus type 2 and type 4 in human lymphocyte cultures.
• Kinetic Analysis of the Decomposition of the KFe3(SO4)2-x(CrO4)x(OH)6 Jarosite Solid Solution in Ca(OH)2 Medium
• Modulation of Aflatoxin B1–Mediated Genotoxicity in Primary Cultures of Human Hepatocytes by Diindolylmethane, Curcumin, and Xanthohumols
• Protective effect of sodium selenite against the genotoxicity of aflatoxin B1 in human whole blood cultures
• Human histone 3 F2 detects RFLPs in inbred mice, cosegregates with H4F2 and does not map to possible syntenic groups on mouse distal chromosomes 1 and distal chromosomes 3.