Genetic variability in the G gene of human respiratory syncytial virus (hRSV) and in the F gene of human metapneuvirus (hMPV) / Variabilidade do gene G do virus repiratorio sicial humano (hRSV) e gene F do metapneumovirus humano (hMPV)

AUTOR(ES)

Luciana Helena Antoniassi da Silva

DATA DE PUBLICAÇÃO

2007

RESUMO

The members of the Paramyxoviridae are enveloped, non-segmented viruses, with negative-sense single stranded genomes. Respiratory syncytial virus (hRSV) is the best characterized agent viral of this group, associated with respiratory diseases in lower respiratory tract. Recently, a new human pathogen belonging to the subfamily Pneumovirinae was identified, the human metapneumovirus (hMPV), which is structurally similar to the hRSV, in genomic organization, viral structure, antigenicity and clinical symptoms. In the first part of the present work the objective is to analyze the genetic variability hRSV isolates circulating in the region of Campinas, in the period April and September 2004, comparing previously obtained sequences from the F and G protein gene of such strains with other partial and complete gene sequences from the homologous genes from isolates identified in other countries. The phylogenetic analysis conducted here allowed us to allocate the isolates belonging to groups A and B. The isolates belonging to the group A showed three clusters, GA2, GA5 and SAA1. The genotype SSA1 was previously identified in South Africa, and there are few reports of such genotype it in the literature. Analyzed sequences belonging to group B were identified in three distinct clusters, GB3 (SAB3) and BA (BAIII). Of the analyzed sequences in the present study, two were identified as belonging to genotype BA the isolates showed 60 nucleotide (nt) duplication at positions 781-840 the gene. In the second part of the thesis the objective was to identify the presence and molecular characterization of hMPV by partially sequencing of the F protein gene in samples collected from children of two university hospitals in the region of Campinas, São Paulo state, Brazil. Based on nucleotide sequences of hMPV F gene, we identified the presence of subgroup B/genotype B1 in our work, similarly to reports in 2004 in Australia. Our samples showed to variants in the same genotype, based on nucleotides sequences

ASSUNTO(S)

genetic variability variabilidade genetica

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