Frequência e diversidade de colifagos somáticos isolados de amostras de água do mar, plâncton e bivalves da baixada santista, canal de São Sebastião e Ubatuba. / Frequency and diversity of somatic coliphages isolated from seawater, plankton and bivalves samples from baixada Santista, Canal de São Sebastião e Ubatuba.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The somatic coliphages (SC) are the better indicator for fecal pollution. In this research, it was obtained the SC abundance in seawater, plankton and bivalves samples collected from Santos, São Sebastiâo and Ubatuba. SC counts were correlated with marine viable bacteria, thermotolerant coliforms, E. coli and intestinal enterococci, and the correlation was negative with the temperature. Highest SC counts were obtained from samples collected at Santos. The frequency of SC families found in seawater and plankton samples were: Siphoviridae (50% and 65.8%), Podoviridae (36% and 15.8%), Microviridae (9% and 15.8%), and Myoviridae (5%, 2.6%), respectively. In bivalves, only Siphoviridae was found. Morphotypes A1 (3%), B1 (63%), C1 (21%) and D1 (13%) were observed. The RFLP and rep-PCR techniques were not discriminatory. 9.6% of coliphages contained genes codifying for thermostable toxin (ST) and/or thermolabil toxin (LT). This study is identifying the coliphages as microbial hazard and giving support to later studies for microbial risk assessment of marine ecosystem.

ASSUNTO(S)

marine ecosystems Água do mar microbiology diversity plâncton califagos somáticos diversidade ecossistemas marinhos região costeira de são paulo bacteriophages somatic coliphages bacteriófagos plankton coastal region of são paulo microbiologia seawater

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