Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria.
AUTOR(ES)
Pelicic, V
RESUMO
Expression in mycobacteria of the structural gene sacB, which encodes the Bacillus subtilis levansucrase, was investigated. sacB expression is lethal to Mycobacterium smegmatis and Mycobacterium bovis BCG in the presence of 10% sucrose. sacB could thus be used as a counterselectable marker in mycobacteria.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=177784Documentos Relacionados
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