Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli.
AUTOR(ES)
Gay, P
RESUMO
A clone bearing the structural gene sacB, coding for the exoenzyme levansucrase, was isolated from a library of Bacillus subtilis DNA that was cloned in phage lambda charon 4A on the basis of the transforming activity of the chimeric DNA. This lambda clone also was found to contain the sacR and smo loci. Subcloning the sacB-sacR region in plasmid pBR325 resulted in a clone which directed levansucrase synthesis in Escherichia coli. The nucleotide sequence coding for the secreted protein was localized on the physical map of the cloned DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=221793Documentos Relacionados
- Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria.
- Conditional suicide system of Escherichia coli released into soil that uses the Bacillus subtilis sacB gene.
- Cloning and Expression in Escherichia coli of the Regulatory sacU Gene from Bacillus subtilis
- Cloning and expression in Escherichia coli of the regulatory sacU gene from Bacillus subtilis.
- Markerless deletions of pil genes in Myxococcus xanthus generated by counterselection with the Bacillus subtilis sacB gene.