Expressão do receptor ativador de NF-kBETA (Rank), Rank ligante (RANKL), e Osteoproterina (OPG) em sítios de reparo ósseo de ratos diabéticos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

To clarify the mechanisms of altered bone reapair in diabetic state, we investigate the RANK, RANKL and OPG expression by immunohistochemistry and RT-PCR in the fracture sites of diabetic rats. A closed fracture was performed on the anatomical left tibia in rats either healthy or made diabetic by alloxan. Histomorphometric analysis of fracture site at 7 days after fracture revealed that diabetic rats (db) have significantly lesser bone and cartilage formation at fracture site in comparison with controls. Parallel with this, the number of RANK and RANKL positive cells were slighly decreased in db group. Furthermore, OPG+ cells were significantly lower in db than control (p=0.05). However, the RANKL/OPG ratio was similar in control (0.074) and db (0.099) at this time. At day 14, the numbers of RANKL and OPG positive cells and the mRNA expression for these markers were increased in control group (P=0.008). Despite lower levels, the RANKL/OPG ratio in db group (1.29) was greater than in controls (0.90) what suggets a favoring of pro-resorptive pathways. Our results demonstrate the expression of consistent markers of hard tissue formation/remodeling activities in sites of fractures. The imbalance of RANKL/OPG expression may contribute to the delay of fracture repair during diabetes course.

ASSUNTO(S)

medicina opg diabetes reparo ósseo rank rank opg facture repair rankl rankl diabetes

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