Experimental evidence for RNA trans-splicing in mammalian cells.
AUTOR(ES)
Eul, J
RESUMO
We present evidence that mammalian cells have the ability to generate functional mRNA molecules by trans-splicing. Rat cells, transformed by an early SV40 DNA fragment (Bst/Bam) synthesize a truncated T antigen (T1 antigen), although the cells do not have a direct sequence homology for the T1 antigen at the DNA level. The Bst/Bam DNA fragment encodes exclusively for the second SV40 T antigen exon (aa 83-708) and contains the entire small t antigen intron. To synthesize the corresponding mRNA (T1 mRNA), the cells utilize a cryptic 5' splice site within the second exon (codons for aa 131/132) as donor site and the upstream small t antigen 3' splice site as the acceptor site. Since these sites are in an inverted order on the pre-mRNA, two Bst/Bam transcripts are required to generate one T1 mRNA molecule. HeLa cell nuclear extracts also performed the trans-splicing reaction in vitro.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=394384Documentos Relacionados
- Localization of an exonic splicing enhancer responsible for mammalian natural trans-splicing
- mRNA 5′-leader trans-splicing in the chordates
- Messenger RNA reprogramming by spliceosome-mediated RNA trans-splicing
- In vitro trans-splicing in Saccharomyces cerevisiae.
- Trans-splicing and alternative-tandem-cis-splicing: two ways by which mammalian cells generate a truncated SV40 T-antigen.