EVALUATION OF THE PROCESSING OF DRY BIOLOGICAL FERMENT FOR GAMMA RADIATION / "Avaliação do processamento de fermento biológico seco por radiação Gama"

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

The developed work had with objectives to demonstrate if it had alteration in the growth of UFC in plate and in the viability of yeasts and total bacteria when dry biological ferment was dealt with by different doses to gamma radiation and under different times storage, to determine the D10 dose for total bacteria and yeasts in this product and to analyzed the processing of this product it promoted some benefit without causing unfeasibility of exactly. The different samples of dry biological ferment had been irradiated at IPEN in a Gammacell – 220 source at 0.5; 1; 2 and 3kGy doses (doserate of 3.51kGy/h). This procedure referring samples to each dose of radiation had been after destined to the microbiological analysis and the test of viability while excessively the samples had been stored the ambient temperature (23ºC). The increase of the dose of radiation caused a reduction in the counting of yeasts growth, of total bacteria growth and also in the frequency of viable yeast cells, demonstrated by FDA-EB fluorescent method. Beyond of radiation the storage time also it influenced in counting reduction of total bacteria and reduction of frequency of viable cells. According with the analysis of simple linear regression, the dose of radiation necessary to eliminate 90% of the yeast population was between 1.10 and 2.23kGy and for the bacterial population varied between 2.31 and 2.95kGy. These results demonstrated clearly the negative points of the application of ionizing radiation in dry biological ferment; therefore the interval of D10 found for total bacteria is superior to found for yeasts. Being thus, the use of this resource for the improvement of the product quality becomes impracticable, since to reduce significantly the bacterial population necessarily we have that to diminish the population of yeasts. With yeasts reduction of we will go significantly to modify the quality and the viability of product.

ASSUNTO(S)

fermento biológico seco radiação gama saccharomyces cerevisiae fermento biológico seco radiação gama saccharomyces cerevisiae

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