Avaliação morfologica e imunohistoquimica dos efeitos agudos da radiação gama em glandulas parotidas de ratos / Morphological and immunohistochemical evaluation of the gamma radiation acute effects on rat parotid glands

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

The aim of this study was to evaluate the morphological and immunohistochemical characteristics of gamma radiation acute effects on rat parotid glands. Twenty-one animals were divided into two groups: control and irradiated ones. The former was composed by non-exposed animals, while the latter was formed by rats which had their necks and heads irradiated with 15 Gy. The parotid glands were excised at 4, 8, 12, 24, 48 and 72 hours after irradiation and, then, were submitted to immunohistochemical methods, for the evaluation of the laminin, and to hematoxylin-eosin staining, for the analysis of the morphology. Laminin staining intensities between irradiated and non-irradiated salivary glands showed no statistically significant differences. Nevertheless, it was observed that laminin thickening and laminin-positive conglomerations demonstrated significant differences between the control and the 72-hour-subgroup (p= 0,0315). The morphological analysis revealed the presence of vacuolation, acinar atrophy and necrosis, especially by 8 and 12 hours after irradiation. Regeneration of the tissue started by 24 hours, but it was not complete at 48 and 72 hours after exposure. The results showed that radiation may be one of the factors that induce extracellular matrix remodeling

ASSUNTO(S)

radiobilogia glandulas salivares radiobiology laminin laminina salivary glands

ACESSO AO ARTIGO

Documentos Relacionados