Evaluation of the impact of orally administered carbohydrates on postprandial blood glucose levels in different pre-clinical models

AUTOR(ES)

Marques, Any de Castro Ruiz, Schiavon, Fabiana Percinoto Monteiro, Travassos, Patricia Batista, Eik, Vanessa Fontana, Godoy, Guilherme, Schamber, Christiano Rodrigues, Bazotte, Roberto Barbosa

FONTE

Braz. J. Pharm. Sci.

DATA DE PUBLICAÇÃO

2016-12

RESUMO

ABSTRACT We developed a pre-clinical model in which to evaluate the impact of orally administered carbohydrates on postprandial blood glucose levels. For this purpose, we compared the effects of different carbohydrates with well-established glycemic indexes. We orally administered (gavage) increasing amounts (0.2, 0.4, 0.6, 0.8, and 1.0 g/kg) of sucrose and lactose to rats which had been fasted for 6 h or 15 h, respectively. In part of the experiments we administered frutose (gavagem). Three different models were compared for measuring postprandial blood glucose levels: a) evaluation of interstitial glucose concentrations by using a real time continuous glucose monitoring system; b) evaluation of glucose levels in blood obtained from the rat tail; c) evaluation of serum glucose levels in blood collected after decapitation. Our results showed that blood obtained from the tails of 15-h fasted rats was the best model in which to evaluate the effect of carbohydrates on postprandial blood glucose levels.

Documentos Relacionados

• Impact of a Pre-Clinical Clinical Skills Curriculum on Student Performance in Third-Year Clerkships
• EVALUATION OF HIGH LEVELS OF SPORTS ACTIVITY AND THE BENEFICIAL EFFECT ON POSTPRANDIAL BLOOD GLUCOSE PROFILES
• A magneto-motive ultrasound platform designed for pre-clinical and clinical applications
• Validating of the pre-clinical mouse model for metastatic breast cancer to the mandible
• Microarray analysis in clinical oncology: pre-clinical optimization using needle core biopsies from xenograft tumors