Esvaziamento gastrico de liquidos em ratos submetidos a ligadura do ducto biliar

AUTOR(ES)
DATA DE PUBLICAÇÃO

1999

RESUMO

The objective of this study was to evaluate gastric emptying (GE) of liquids in rats with bile duet ligature. In the first phase (step 1), 18 animals were distributed in 2 subgroups: with bile duet ligature (L) and sham (S). After two weeks receiving ration with 10% fat, the obstruction model of the biliary path was evaluated as to morphology (macroseopic and histological), bioehemistry (plasma levels of bilirubin, aminotransferases, alkaline phosphatase, amylase and y-glutamil-transpeptidase) and function (steatocrit acid of the rats eeeo-appendix content). In the second phase (step 2), 132 animals were distributed in 5 groups according to the liquid test meal (LTM) Saline group (SG), in which the LTM was 0.9% NaCI, with 36 animals divided into control C, S and L subgroups, with 12 animals in each, the mayonnaise groups at 3% and 6% (MG3 and MG6) and glucose groups at 5% and 10% (GG5 and GGlO), which were divided into subgroups S and L, also with 12 animals in each. In this phase, after 2 weeks receiving commercial ration, Labina (Purina), gastrie retention (GR) for the 5 meals tested was determined and the procedures of phase I were repeated, with the exception of the steatoerit acid. GE was studied in animals undergoing 24 hour fasting, receiving water ad libitum; LTM was administered by standard technique, using the volume of 2mVg the animal s weight. For the statistical analysis, the non parametric Mann-Whitney (K=2) and Kruskal- Wallis (K:::: 3) test was used, having established that the value of a.=0.05. As there was a significant difference in the Kruskal -Wallis test, the multiple comparisons test was applied in the differentiation between the pairs (a. = 0.02). The results of phase I demonstrated the efficacy of the bile duet ligature, as to the aspects analyzed. The following facts stand out: 1- no significant differences were observed in the levels of serie amylase when groups were compared; 2- the animals with bile duet ligature presented significantly greater values of steatocrit (median = 58.5%) in relation to the simulation group (median = 8%). The results of phase II demonstrate significantly lower weight gain in the animais with bile duct ligature, as well as significantly greater values of humid and dry weight, and liquid content of the liver of these in relation to the simulated animais. The results of the biochemical determinations performed in this phase did not differ from those obtained in phase I. The emptying of the saline LTM was not influenced by the bile duct ligature (median of GR = 38.5%) in relation to the control group (median of GR = 36.3%) and simulation group (median ofGR = 36.9%). As to the LTM of mayonnaise at 3 and 6%, a significantly lower GR was observed in the groups with ligature (median of GR of MG3 = 30.4% and MG6 = 35.4%), when compared to the respective simulation groups (median of GR ofthe MG3 = 38.6% and of MG6 = 47.3%). On the other hand, the GR of LTM of glucose at 5% did not show significant difference in the animais with bile duct ligature (median of GR of the GG5 ",;45.2%), in relation to the sham (median GR of the GG5 = 41,5%), however, in the GR of the LTM glucose at 10%, significantly greater values )were observed in the animais with bile duct ligature (median of GR of GGI0 = 66.8%), when compared to the respective simulation groups (median ofGR ofGGlO = 53.6%). Thus, the conclusion is that in rats the absence of biliary flux to the small intestine increased the GE of fats and retarded the GE o(glucose to 10%, when macronutrients were administered in liquid meal form.

ASSUNTO(S)

gastroenterologia sistema gastrointestinal - motilidade

ACESSO AO ARTIGO

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