Estudo do efeito de gangliosideos sobre a incidencia e morbidade do diabetes mellitus em camundongos NOD/Uni (non-obese diabetic)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2000

RESUMO

Diabetes mellitus type l(DM-l) is an autoimmune disease that results ITom destruction and dysfunction of pancreatic _-cells. This process, known as insulitis, is dependent on the presence of a mononuc1ear cells infiltrate in islets of Langerhans. Non obese diabetic (NOD) mouse is an experimental model of DM-l that develops spontaneous diabetes between the 4th and 6th month of life, similar to human disease. The aim of the present study was to verify the effects of the treatment with gangliosides on the incidence of spontaneous and cyc1ophosphamide accelerated diabetes model (CY-DM) in NOD mice. The animaIs were treated daily with a mixture of gangliosides GMl(21 %),GDla (40%), GDlb(16%),GTlb(19%) (150 mg/kg) between 4th and 17th or 24th week oflife and monitorized for the development of DM-l with urinary and blood glucose concentration. After sacrifice, morphologic analysis, inc1uding quantification and c1assification of insulitis degree was performed. The results show that the prevalence of diabetes was lower in gangliosides treated animaIs, even in those submitted to cyc1ophosphamide (CY-DM-l). These findings are in accordance with morphologic analysis that demonstrates that treated animaIs present lower degrees ofinsulitis in both spontaneous and CY-DM-l, results that could be also observed 8 weeks after the interruption of treatment. These findings reinforce the hypothesis of the immunomodulatory action of gangliosides, probably related to the inhibition of T cells proliferation or cellular signaling. In conc1usion, treatment of NOD mice with gangliosides could decrease insulitis degree and the prevalence of DM in this experimental model. However, new studies, directed to analyze the molecular mechanisms involved in these processes are needed in order to elucidate the specific mechanisms of immunomodulatory action of gangliosides

ASSUNTO(S)

camundongo diabetes

ACESSO AO ARTIGO

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