Estudo de um sistema de análise de preferências climáticas para animais convencionais de laboratório por tecnologia de ventilação microambiental e avaliações biológicas. I. Avaliação da eficiência de um protótipo, para o estudo de parâmetros ambientais, resultados parciais verificados em ratos (Rattus norvegicus) / A climatic chamber employing Intracage Ventilation System to evaluated house condition of laboraroty animals and to biological assays in thermoregulation studies. Partial evaluation in rats (Rattus norvegicus)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Projects concerned with laboratory animals facilities usually adopt narrow range of ventilation and climatization variables to assure the define ambient. Such projects are concerned to the animal room (macro-environmental ambient) and this technology have a limitated operation to reach the micro-environmental (animal cages) ambients. The development of the intracage ventilation systems (ICV), which operating by an air influx inside the cages, beside the new technology on thermoelectric modules, thermosensitive and thermocontrolers materials, allows an accurate control of the microclimatic variables for animal enclosure. For this purpose we developed an experimental prototype, a climatic preference chamber (CPC) that allows to laboratory animal (e.g. rats) choose their preferred ambient under a different temperature conditionated chambers by the ICV thecnology, simulating the animal cage enclosure condition. That technology is characterized by delivery of air insufflation of 0.3 m/s, and regulated with differents temperatures in each chamber (22, 26, 30 and 34oC). The exploratory behavior of the rat at multiplechoise preference test was registered by Ethovision Color-Pro 2.3® system. This system allowed to the analysis of the climatic preference defined by the parameter time spent in different microclimates conditions. The analyses of the exploratory behavior demonstrated that rats spent much time in smaller temperature microclimates, as a 22 and 26 oC, indicating a preference for cooler ambients, in spite of the ICV air speed of 0,3m/s. The aim of this work is simulate laboratory animal housing condition to study the microclimate preference and also in termoregulation studies with laboratory rodents assays, like neurotoxins of the the spider Phoneutria nigriventer venom, that is an important tool to biochemical studies

ASSUNTO(S)

câmara climática climatic chamber microenvironmental ventilation preference test ratos rats temperatura temperature teste de preferência ventilação microambiental

ACESSO AO ARTIGO

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