Estudo de dois genes da imunidade inata (MBL â 2 e o receptor CCR5) na transmissÃo materno infantil do HIV-1

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The Acquired Immunodeficiency Syndrome (AIDS) is a disease caused by Human immunodeficiency Virus (HIV) and is characterized by cell destruction of immunologic system, as macrophages and T CD4. According statistics by Joint United Nations Program on HIV/AIDS (UNAIDS) approximately 42 millions of people are living with HIV worldwide and only in Brazil there are 600 thousand people are living with HIV. Agreement of the mechanisms of the innate immunity is very important to control both infection and replication. This work had as objective to consider a simple, fast and efficient methodology to correlate through the genotyping of two genes related in immune innate response (CCR5 and MBL-2), the sucessibility or resistance to infection by HIV-1, and the risk of through vertical transmission in Brazilian other/children pairs, infectados for the HIV-1, using melting temperature assay as method for the detection of single-nucleotide polymorphisms. Ours results to genotyping of the MBL-2 gene are in agreement with the literature data which indicate that the presence of the allele 0 confers a relative risk of 1.37 for HIV-1 infection through vertical transmission. In this work, we also analyzed the frequencies of four CCR5 promoter polymorphisms, and of the Δ32 deletion in Brazilian HIV-1 + and HIV-1 â children. CCR5 polymorphisms were detected by PCR amplification and direct sequencing. Although no significative divergence was found for CCR Δ32, CCR5-59356-C/T and CCR5-59653 C/T polymorphisms, the frequency of CCR5-59353-T/C and CCR5-59402-A/G genotypes differed among HIV+, HIV− and HC children. The presence of the CCR5-59353-TT genotype indicated a trend for increased risk of vertical transmission of HIV-1 infection in Brazilian children, while the presence of the CCR5-59402-AA genotype was suggestive of a protective effect against HIV-1 vertical transmission

ASSUNTO(S)

ccr5 imunidade inata hiv-1 transmissÃo materno infantil mbl ciencias biologicas

ACESSO AO ARTIGO

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