EpsR Modulates Production of Extracellular Polysaccharides in the Bacterial Wilt Pathogen Ralstonia (Pseudomonas) solanacearum

AUTOR(ES)

Chapman, Matt R.

FONTE

American Society for Microbiology

RESUMO

Ralstonia solanacearum is the causal agent of bacterial wilt of many agriculturally important crops. Exopolysaccharide synthesized by products of the epsI operon is the major virulence factor for R. solanacearum. Expression of epsI has been demonstrated to be under the control of several proteins, including several two-component regulators. Overexpression of EpsR was found previously to reduce the amount of synthesis specifically from the epsI promoter. Here we present data that a single chromosomal copy of epsR activates the epsI promoter, suggesting that EpsR is a concentration-dependent effector of epsI gene expression. Furthermore, the ability of EpsR to modulate epsI expression is dependent on the phosphorylation state of EpsR. Gel mobility shift assays suggest that EpsR can specifically bind the epsI promoter and that this binding requires a phosphorylated form of EpsR.

Documentos Relacionados

• Lipopolysaccharide-Defective Mutants of the Wilt Pathogen Pseudomonas solanacearum
• A two-component system in Ralstonia (Pseudomonas) solanacearum modulates production of PhcA-regulated virulence factors in response to 3-hydroxypalmitic acid methyl ester.
• Lipopolysaccharide Composition of the Wilt Pathogen, Pseudomonas solanacearum: CORRELATION WITH THE HYPERSENSITIVE RESPONSE IN TOBACCO 1
• Avaliação da resistência de clones e cultivares de batata à murcha bacteriana (Ralstonia solanacearum)
• Essential oil of mustard an d bacterial wilt on experimental control in tomato (Ralstonia solanacearum): effectiveness and toxicity to the pathogen