Enzyme-linked immunosorbent assay for detection of human antibodies to Salmonella typhi Vi antigen.
AUTOR(ES)
Barrett, T J
RESUMO
An enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to Salmonella typhi Vi antigen in human serum, and the results were compared with those from a previously described hemagglutination assay (HA). The ELISA detected Vi antibodies at a titer of greater than or equal to 20 in 40 (52%) of 77 sera from typhoid fever patients, whereas the HA gave titers of greater than or equal to 20 in 35 (47%). Determination of titers of serum specimens from 170 persons without typhoid fever revealed Vi antibody titers of greater than or equal to 20 in 4 (2.3%) by the ELISA and 3 (1.7%) by the HA. Unlike the sensitized erythrocytes used in the HA, the ELISA reagents have a shelf life of greater than or equal to 1 year. The ELISA may be preferred by some laboratories, especially those already performing other ELISA tests.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=272705Documentos Relacionados
- Enzyme-linked immunosorbent assay for detection of Salmonella typhi protein antigen.
- Enzyme-linked immunosorbent assay for detection of Salmonella typhi Vi antigen in urine from typhoid patients.
- Enzyme-linked immunosorbent assay for detection of Streptococcus pneumoniae antigen.
- Comparison of diffusion-in-gel enzyme-linked immunosorbent assay with conventional serological methods for detection of class-specific antibodies to Salmonella typhi O antigen.
- Development and evaluation of an enzyme-linked immunosorbent assay for serum Vi antibodies for detection of chronic Salmonella typhi carriers.
