Development and evaluation of an enzyme-linked immunosorbent assay for serum Vi antibodies for detection of chronic Salmonella typhi carriers.
AUTOR(ES)
Losonsky, G A
RESUMO
An enzyme-linked immunosorbent assay (ELISA) measuring, in serum, immunoglobulin G (IgG), IgM, and IgA to Vi capsular polysaccharide antigen that was tyraminated (Vi-Tyr) to increase its binding efficiency to microtiter plates was compared with the standard passive hemagglutination assay (PHA) as a screening test for chronic Salmonella typhi carriers. Initially, three populations were evaluated: 22 healthy U.S. adults, 17 young Chilean adults with acute typhoid fever, and 51 Chileans who had bacteriologically confirmed S. typhi chronic carriage. IgG-specific Vi-Tyr antibodies were preferentially present in the S. typhi chronic carrier state. A total of 44 of 51 (81%) chronic carriers, 0 of 22 (0%) healthy U.S. adults, and 2 of 17 (12%) Chileans with acute typhoid fever had reciprocal IgG Vi-Tyr ELISA antibody titers in serum of greater than or equal to 200. The IgG Vi-Tyr ELISA was then compared with the PHA as a screening test for chronic carriers in 141 Chilean female food handlers. One woman was serologically incriminated as a carrier by both the IgG ELISA and PHA; her coprocultures were positive for S. typhi. One other woman, identified as a carrier by PHA, was negative by culture and IgG ELISA. The IgG Vi-Tyr ELISA is as sensitive as the PHA (86 versus 76%) and as specific (95 versus 95%) in screening for chronic carriers.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=269467Documentos Relacionados
- Enzyme-linked immunosorbent assay for detection of human antibodies to Salmonella typhi Vi antigen.
- Enzyme-linked immunosorbent assay for detection of Salmonella typhi protein antigen.
- Enzyme-linked immunosorbent assay for detection of Salmonella typhi Vi antigen in urine from typhoid patients.
- Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to bovine leukemia virus in serum and milk.
- Interlaboratory evaluation of indirect enzyme-linked immunosorbent assay, antibody capture enzyme-linked immunosorbent assay, and immunoblotting for detection of immunoglobulin M antibodies to Toxoplasma gondii.