Effects of GM1 administration on autoimmune diabetes modulation and cytokines expression, Nerve Growth Factor and TrkA receptor in NOD mice (non obese diabetic) / Avaliação dos efeitos da administração do gangliosideo GM1 na modulação do diabetes mellitus autoimune e expressão de citocinas, Nerve Growth Factor e seu receptor TrkA em camundongos NOD (non obese diabetic)

AUTOR(ES)

Karla Priscila Vieira Ferro

DATA DE PUBLICAÇÃO

2007

RESUMO

The strain of NOD mice (non obese diabetic) spontaneously develops diabetes mellitus type 1 (DM-1) with strong similarity to the observed in humans. In this model, the diabetes manifestation occurs among 12th and 24th weeks of life, with presence of pancreas-specific autoantibodies. Great part of the cells are CD4+ and CD8+T cells, and even so NK cells, lymphocytes B, dendritics cells and macrophages also can be identified in the injuries, the development of the disease is essentially dependent of autoreactive CD4+ and CD8+ T cells. It was demonstrated that ? - pancreatic cells express NGF functional receptors and that this neurotrophin induces morphological and physiological modifications in pancreatic ßcells, including stimulation in insulin secretion. The inquiries of therapies for the DM-1 based on the intervention on the immune system disclose that these can be promising strategies to hinder the installation and/or evolution of the disease. In this context, we investigate the effect of GM1 administration on the incidence of DM-1 and insulitis in NOD mice, cytokines expression, NGF and its high affinity receptor TrkA. Our results suggest that administration of GM1 in the dose of 100mg/kg/dia in female NOD mice from 4ª week of life are capable to reduce the index of inflammatory infiltrated and consequently the expression of diabetes, down-modulating the inflammatory infiltrated as well as the gene expression of pro-inflammatory cytokines (IL-12, IFN-γ TNF-α and IL-1ß), besides increasing the gene and protein expression of NGF and TrkA, that can act as regulating of ß cell - survival in way to inhibit apoptosis of this cell.

ASSUNTO(S)

gangliosides nod mice camundongos nod citocinas gangliosideos diabetes mellitus type 1 diabetes mellitus tipo 1 cytokines

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