Effects of calcium channel blocker (Verapamil) on human dermal fibroblasts. / Efeitos do bloqueador do canal de cálcio (Verapamil) sobre fibroblastos dérmicos humanos.
AUTOR(ES)
Ricardo Frota Boggio
DATA DE PUBLICAÇÃO
2008
RESUMO
Excessive scar tissue (keloids and hypertrophic scars) is a defective wound healing process characterized by overproduction of extracellular matrix. In the present study human dermal fibroblasts treated with 50 mM verapamil changed their normal spindle-shaped morphology to stellate/rounded and showed discrete reorganization of microfilaments We hypothesized that these effects would be associated to lower levels of cytosolic Ca2+. Indeed, short time loading with calcium green confirmed that verapamil-treated fibroblasts exhibited lower intracellular calcium levels. We also observed that verapamil decrease cellular proliferation by 64.4%, increase the secretion of MMP1 and decrease synthesis of collagen in cultured fibroblasts. This alterations induced by verapamil are not associated with cytotoxic effects. The cellular calcium metabolism appears to regulate extracellular matrix production and so those hypertrophic disorders of wound healing (keloids and hypertrophic scars) may respond to therapy with calcium antagonist drugs (verapamil).
ASSUNTO(S)
collagenase collagen verapamil human fibroblasts actina cálcio citosólico cytosolic calcium colagenase colágeno fibroblastos humanos verapamil actin
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