Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans

AUTOR(ES)

Kamath, Ravi S

FONTE

BioMed Central

RESUMO

RNAi can be achieved by feeding worms Escherichia coli expressing dousble-stranded RNA corresponding to a specific gene. An optimized feeding method is presented that results in phenotypes at least as strong as those produced by direct injection of RNA for embryonic lethal genes, and stronger for genes with post-embryonic phenotypes.

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