Double-stranded RNA-mediated gene silencing in fission yeast

AUTOR(ES)

Raponi, Mitch

FONTE

Oxford University Press

RESUMO

Double-stranded RNA (dsRNA) can specifically inhibit gene expression in a variety of organisms by invoking post-transcriptional degradation of homologous mRNA. Here we show that dsRNA-mediated gene regulation also occurs in the fission yeast Schizosaccharomyces pombe. We present evidence that: (i) reporter gene silencing is significantly enhanced when additional non-coding sense RNA is co-expressed with antisense RNA; (ii) expression of a panhandle RNA also silences target gene expression; (iii) expression of dsRNA is associated with siRNAs; (iv) a novel host-encoded factor which enhances antisense RNA gene silencing also enhances panhandle RNA-mediated gene inhibition. Both the exogenously introduced lacZ and c-myc genes are shown to be susceptible to dsRNA- mediated gene silencing in this model. Taken together, these data indicate that RNA-mediated gene silencing can occur through a RNAi-like mechanism in fission yeast.

Documentos Relacionados

• Double-Stranded RNA-Mediated Interference with Plant Virus Infection
• Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans
• RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans
• Dominant genetic screen for cofactors that enhance antisense RNA-mediated gene silencing in fission yeast
• Packaging in a yeast double-stranded RNA virus.